External Anatomy


Purpose:     To observe the characteristics that identify a frog as an amphibian.  To relate the structures of the frog to their functions.

Procedure: Place the frog on the dissecting pan, ventral side down.  Locate the structures identified in the data section that follows.

Data:  General External Features

The body of a frog can be divided into a head, which extends posteriorly to the shoulder region, and a trunk. Notice that a distinct neck is absent.  This is a retention of a characteristic of fishes, for which an independent motion of the head and trunk would be disadvantageous during swimming.  The appendages, forearms and hind legs, allow the frog to move about on land.  The large webbed hind feet aid in swimming. Label the head, trunk and appendages on the diagram below.

Head Section

A large mouth, a pair of nostrils, or external nares (singular naris), and the eyes will be recognized on the head.  The upper eyelid is a simple fold of skin, but the lower lid is a transparent membrane which can be drawn across the surface of the eyeball.  This is the nictitating membrane, which protects the eye when the frog is under water and keeps it moist when the frog is on land.  The disc-shaped area posterior to each eye is the ear drum, or tympanic membrane. Look carefully at the top of the head between the eyes, and you may see a small, light brow spot, which has about the diameter of a pin.  This is a remnant of a median, light-sensitive eye that characterized primitive group of fishes and amphibians.  Label the structures, in blue print, on your drawing below.

Trunk Section

The combined orifice of the digestive and urogenital tracts, the cloacal aperture, is at the posterior end of the trunk just dorsal to the junction of the hind legs.  The anus of human beings and other mammals, since it is the opening of just the digestive tract, is only partly comparable to the cloacal aperture.

Frogs’ skin is exceptionally thin as well as vascular, and it is kept moist by the secretions of numerous microscopic mucous glands.  These attributes make it an effective respiratory membrane.  About one-third of the environment of gases between the blood and environment takes place through the skin.  This is referred to as cutaneous respiration.  However, the characteristics of the skin also permit considerable exchange of water across it-a loss of body water when on land, a gain of water when in an aquatic environment.  Most of the water that the frog needs is absorbed by osmosis through the skin.  The small, branching, dark spots which you see with a microscope are the pigment cells, or chromatophores.  These particular cells contain granules of a dark pigment known as melanin. The granules can migrate out to the processes of the chromatophores, making the frog darker, or they can concentrate near the center of the cells, giving the frog a lighter color.  Other pigment cells contain a yellow pigment, and others contain refractive crystals of guanine which disperse light to give a blue effect.  The combination of yellow and blue causes the green colors.  Label the structures, in blue print, on your drawing below. Color the frog diagram to represent how the pattern conceals the frog in its natural habitat.

Appendages

The legs of a frog have the same parts as our own.  In the front leg, or pectoral appendage, notice the upper arm, the elbow, the forearm, the wrist joint, and the hand.  How many fingers are on the front hand?.  Are they webbed? Although the finger closest to the body is comparable to our second digit, it is often called the thumb because it is stouter than the others.  During the breeding season, the thumb is particularly stout and darkly pigmented - an example of sexual dimorphism.  In the hind limb, or pelvic appendage, observe and label the thigh, the knee, the shank, the ankle joint, and the very long foot.  Two elongated ankle bones lie within the proximal part of the foot. Describe the foot. How many toes are on the foot? Are the toes webbed? The first toe is the smallest and the one closest to the body, and it is comparable to our great toe.  Technically it is called the hallux, and the small spur at its base is the prehallux.  The prehallux is much larger in toads, which use their hind feet in burrowing. Label the structures, in blue print, on your drawing below.

Frog,s Sex:

To determine the frog’s sex, look at the hand digits, or fingers, on its forelegs. A male frog usually has thick pads on its "thumbs," which is one external difference between the sexes, as shown in the diagram below. Male frogs are also usually smaller than female frogs. Male frogs also have a larger tympanic membrane than females. Observe several frogs to see the difference between males and females.
 

Analysis Questions:

  1. What are the three major divisions of the frog external anatomy?
  2. The color pattern of the frog is said to be cryptic. Explain how it's color conceals the frog in its natural habitat. Compare the colors of the dorsal and ventral surfaces of the frog. Does the color on the ventral side help conceal the frog? How?
  3. How can you determine the sex of your frog? Give three examples of sexual dimorphism.
  4. How does the frogs external anatomy compare to the human? What similarities and differences do you observe?

Conclusion:

What did you learn about the frog by observing the external anatomy. What special structures does the frog have that make it uniquely adapted to it's environment? Of what adaptive value is each of these structures to the frog?