BIOLOGY LAB
Food Chemistry
pH Analysis

Introduction:

A liquid may be an acid, base, or neutral. The degree of acidity or basicity can be measured by using the pH scale. The scale is divided into three areas: Acid (readings below 7), neutral (reading of 7), and basic (readings above 7). Each division either increase or decreases the pH of a substance 10 times. The pH of 5 is ten times more acidic than a pH of 6. Water has a pH of 7 but when it mixes with air the suspended materials will either raise or lower its pH. Acid Rain is an example of this type of reaction.

Objective:

The student will determine the pH of various substances and will also determine how some of these substances can affect the environment.

Materials:

  • pH paper and Chart or Indicator Solution
  • Paper Towels
  • 1 depression plate
  • Lemon Juice
  • Cola
  • Food Samples
  • Distilled Water
  • Baking Soda
Procedure:
  • Usie a spot plate.
  • Put 2 drops of each sample in one cell of the spot plate
  • Hypothesize whether the liquid would be acid or base.
  • Add 2 drops of Indicator to each sample.
  • Record this on the data table below.
  • Compare the color of the Indicator with that on the pH chart.
  • Record your observation on the chart below.
  • Repeat the procedure with the rest of the slides.
ooSampleoo
Hypothesis
ypH
Acidic
Basic
pNeutralp
 
dd     
 
    dd     
    dd dd    
 
 
 
 
 
ssssss
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

Analysis:

1. Which of the liquids had the lowest pH ?
 

2. Which of the liquids had the highest pH?
 

3. Which of the liquids were closest to being neutral?
 

4. If the pH of a sample was 3 how many times more acidic is it than a solution with a pH of 6?