Gene Linkage and Crossing Over


Purpose: To determine patterns of heredity using experimental results from fictitious insects.

Procedure: Study the table below. Show the Genetic Cross for each of the problems. Use the symbols given in each question to diagram each of the crosses using a Punnett Square. Answer the Analysis and Conclusions questions at the end of the lab.

Traits
Possible Phenotypes
Body Color Blue White
Eye Color Green Brown
Antennae Long Short
Bristles Straight Curly
Number of Chromosomes 2 Pairs

 

Cross #1
Parents
White-Bodied Male X Blue-Bodied Female
F1
All Blue-Bodied
F2
62 Blue-Bodied
21 White-Bodied

 

Cross #2

Parents
Blue-Bodied, Green-Eyed Male
X
Blue-Bodied, Green-Eyed Female
F1
21 Blue-Bodied, Green-Eyed
7 White-Bodied, Green-Eyed
8 Blue-Bodied, Brown-Eyed
2 White-Bodied, Brown-Eyed

 

Cross #3

Parents
Blue-Bodied, Short-Antennaed Male
X
White-Bodied, Long-Antennaed Female
F1
All Blue-Bodied, Short-Antennaed
F2
66 Blue-Bodied, Short-Antennaed
19 White-Bodied, Long-Antennaed
1 Blue-Bodied, Long-Antennaed
1 White-Bodied, Short-Antennaed

 

Cross #4

Parents
Curly-Bristled, Green-Eyed Male
X
Straight-Bristled, Brown-Eyed Female
F1
All Curly-Bristled, Green-Eyed
F2
75 Curly-Bristled, Green-Eyed
26 Straight-Bristled, Brown-Eyed
1 Curly-Bristled, Brown-Eyed
2 Straight-Bristled, Green-Eyed

Analysis and Conclusions:

  1. Which traits are dominant in this insect?
  2. How many linkage groups are there in this insect?
  3. Which genes are linked? What evidence led you to this conclusion?
  4. Which of Mwndel's Laws are demonstrated by Cross #1? Cross #2?
  5. Are any of the listed traits sex-linked? Explain your answer.
  6. The following cross-over percentages were determined:
    • 23% between the gene for bristles and the gene for eye color.
    • 16% between the gene for body color and the gene for antennae.
    Use this information to draw chromosome mapd for both pairs of chromosomes in a completely heterozygous insect.